Jackson|二抗009-090-050*

ChromPure是我們來(lái)自非免疫動(dòng)物血清的高純度蛋白質(zhì)的商品名。

物理狀態(tài)：冷凍干燥的固體
存儲(chǔ)：
儲(chǔ)存和再水化：將冷凍干燥的固體儲(chǔ)存在2-8°C。用體積的dH2O再水化，如果不清楚則離心。在使用當(dāng)天準(zhǔn)備工作稀釋液。產(chǎn)品在2-8℃下作為未稀釋的液體穩(wěn)定約6周。
再水化后的長(zhǎng)期儲(chǔ)存：等分并在-70℃或更低溫度下冷凍。避免反復(fù)凍融?；蛘?，加入等體積的甘油（ACS級(jí)或更好），終濃度為50％，并在-20°C下儲(chǔ)存為液體。
失效日期：補(bǔ)液之日起一年。如果測(cè)試結(jié)果對(duì)于預(yù)期用途是可接受的，則可以延長(zhǎng)失效日期。

純度：基于抗原濃度為20mg / ml的免疫電泳，針對(duì)山羊抗人全血清的沉淀模式與針對(duì)山羊抗人轉(zhuǎn)鐵蛋白的沉淀模式相同。沒有檢測(cè)到針對(duì)山羊抗人IgG（H + L）的沉淀素系。
緩沖液：0.01M磷酸鈉，0.25M NaCl，pH 7.6
穩(wěn)定劑：15毫克/毫升牛血清白蛋白（無(wú)IgG，無(wú)蛋白酶）
防腐劑：0.05％die氮化鈉

Jackson|二抗009-090-050*

Fluorescein (FITC)

Amax: 492 Emax: 520nm 

FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. A better choice for many applications involving FITC is Alexa Fluor® 488 because it is brighter and more photostable.